Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated HJURP in HeLa nuclear extract (Ana Losada, CNIO, Madrid, Spain).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot immmunoprecipitated HJURP in HEK293 cell lysate (Ana Losada, CNIO, Madrid, Spain).

Detect Holliday junction recognition protein using this mouse monoclonal antibody, Anti-HJURP Antibody, clone 144K/B1 validated for use in western blotting & IP.

General description

Holliday junction recognition protein (HJURP) is also known as 14-3-3-associated AKT substrate (FAKTS), Fetal liver-expressing gene 1 protein (FLEG1) and Up-regulated in lung cancer 9 (URLC9). HJURP is a centromeric protein that acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. HJURP is highly expressed in the thymus, testis, and in many lung cancer samples with lower levels found in placenta, small intestine, liver, skeletal muscle, and colon.

Immunogen

Recombinant protein corresponding to human HJURP.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HJURP in 10 µg of HEK293 cell lysate.

Target description

~90 kDa observed